Coding

Part:BBa_K1796012:Design

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)


nifX promoted from Paenibacillus sp. WLY78


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 325
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 325
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 325
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site（one PstI） was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.


Source

NifX(BBa_K1796012) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.

References